TERMINAL RESPIRATION IN PSEUDOMONAS FLUORESCENS: COMPONENT ENZYMES OF THE TRICARBOXYLIC ACID CYCLE

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Abstract
Chemical and enzymatic analyses of substrate and product demonstrate that the following enzymatic reactions are carried out by cell-free prepns. of Pseudomonas fluorescens: (1) Citrate[forward arrow] alpha-ketoglutarate, (2) alpha-keto-glutarate [forward arrow] succinate, (3) citrate [forward arrow] succinate, (4) acetate + oxalacetate [forward arrow] citrate, (5) cis-aconitate [forward arrow] citrate, and (6) di-isocitrate + triphosphopyridine nucleotide [forward arrow] alpha-ketoglutarate + CO2 + triphosphopyridine nucleotide H2- The enzymes concerned in the reactions appear to be present at corresponding levels when either acetate or citrate constitutes the sole source of C. The adaptive patterns of the intact cells do not permit the conclusion that the tricarboxylic acid cycle represents a significant pathway of terminal oxidation in these organisms.

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