The occurrence of the tricarboxylic acid cycle in yeast

Publisher Website
Google Scholar
Abstract
A cell-free extract of baker''s yeast rapidly metabolizes citrate. For max. activity in the fresh non-dialyzed extract adenosinetriphosphate [ATP] and Mg are required. Even after thorough dialysis there remains a citrate-metabolizing system which is not affected by cyanide. ATP and Mg are now without effect unless boiled yeast juice is also added. Yields of alpha-keto-glutarate approaching the theoretical are obtained from citrate in the dialyzed extract. The further breakdown of alpha-keto-glutarate to oxaloacetate and pyruvate is not inhibited by arsenicals. As similar results could be obtained with a pure sample of Saccharomyces cerevisiae grown in the laboratory, it is concluded that the tricarboxylic acid cycle represents a significant pathway of substrate oxidation in yeast.