Inhibition of Liver Cancer Cell Proliferation and Migration by a Combination of (-)-Epigallocatechin-3- Gallate and Ascorbic Acid
- 1 January 2003
- journal article
- research article
- Published by Taylor & Francis in Journal of Chemotherapy
- Vol. 15 (6) , 591-595
- https://doi.org/10.1179/joc.2003.15.6.591
Abstract
A mixture of (-)-epigallocatechin-3-gallate (EGCG) and ascorbic acid exhibited 73.2% inhibition of SMMC-7721 cell proliferation in a soft agar colony formation assay, which was much higher than EGCG (40.4%) or ascorbic acid (12.4%) alone. In the cell migration assay, the mixture also significantly suppressed the migration of SMMC-7721 cells by 65.9% while EGCG and/or ascorbic acid did by 28.9% and 18.7%, respectively. Ascorbic acid was able to enhance the antioxidant activity of EGCG by decreasing the intracellular oxidative stress according to fluorographic analysis of oxidative stress. In conclusion, the combination of EGCG and ascorbic acid can strongly suppress the proliferation and metastasis of liver cancer cells, possibly with a mechanism associated with the scavenging of reactive oxygen species. All these events add to our knowledge of liver cancer chemotherapy.Keywords
This publication has 16 references indexed in Scilit:
- Inhibitory effect of epigallocatechin gallate on adhesion of murine melanoma cells to lamininCancer Letters, 2001
- Tumor gelatinases and invasion inhibited by the green tea flavanol epigallocatechin-3-gallateCancer, 2001
- Inhibition of melanoma growth and metastasis by combination with (−)‐epigallocatechin‐3‐gallate and dacarbazine in miceJournal of Cellular Biochemistry, 2001
- Enhanced Invasion of Tax-Expressing Fibroblasts into the Basement Membrane Is Repressed by Phosphorylated Ascorbate with Simultaneous Decreases in Intracellular Oxidative Stress and NF-κB ActivationAntioxidants and Redox Signaling, 2000
- Effects of green, oolong and black teas and related components on the proliferation and invasion of hepatoma cells in cultureCytotechnology, 1999
- Stabilizing Effect of Ascorbic Acid on Green Tea CatechinsJournal of Agricultural and Food Chemistry, 1998
- Hydrogen peroxide (H2O2) Increases the Steady-State mRNA Levels of Collagenase/MMP-1 in Human dermal FibroblastsFree Radical Biology & Medicine, 1997
- The implications of angiogenesis for the biology and therapy of cancer metastasisCell, 1994
- Effect of reactive oxygen intermediates on the in vitro invasive capacity of tumor cells and liver metastasis in miceInternational Journal of Cancer, 1993
- Elevated levels of the α5β1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cellsCell, 1990