Flow cytofluorometric analysis of the uptake of the fluorescent fatty acid pyrene-dodecanoic acid by human peripheral blood cells
Open Access
- 1 November 1988
- Vol. 9 (6) , 525-528
- https://doi.org/10.1002/cyto.990090603
Abstract
The fluorescence activated cell sorter (FACS) was used for measuring the uptake of the fluorescent fatty acid derivative 12-(1-pyrene) dodecanoic acid (P12) by human peripheral blood cells. The results indicate that blood cells differ widely in their ability to take up P12, with polymorphonuclear cells showing the greatest uptake, followed by lymphocytes, platelets, and RBCs. These differences in P12 uptake provide a potential additional parameter for differential cell counting. Using the ability of the FACS to “gate out” nonrelevant cells, it was possible to measure the rate of P12 uptake by each respective cell type even when admixed with other cells. Thus elaborate physical separation procedures could be avoided, and contaminating cells did not influence the results. Differences in P12 uptake were also utilized to separate blood cells into pure subpopulations of specific cell types.Keywords
This publication has 15 references indexed in Scilit:
- Use of the fluorescence activated cell sorter for studying uptake of fluorescent fatty acids into cultured cellsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- Uptake of fluorescent fatty acids by erythroleukemia cellsExperimental Cell Research, 1986
- Transport of Fluorescent Fatty Acids into Cultured Cells: Analysis by the Fluorescence Activated Cell SorterPublished by Springer Nature ,1986
- Induction of lipid storage in cultured leukemic myeloid cells by pyrene-dodecanoic acidBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- Transport of fluorescent derivatives of fatty acids into cultured human leukemic myeloid cells and their subsequent metabolic utilizationBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- Immunofluorescent analysis of blood cells by flow cytometryInternational Journal of Immunopharmacology, 1981
- Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood.Proceedings of the National Academy of Sciences, 1980
- SPECTRAL MATCHING OF CLASSICAL CYTOCHEMISTRY TO AUTOMATED CYTOLOGYJournal of Histochemistry & Cytochemistry, 1974
- THE HEMALOG D WHITE CELL DIFFERENTIAL SYSTEMJournal of Histochemistry & Cytochemistry, 1974
- Volume Distribution and Separation of Normal Human Leucocytes.Experimental Biology and Medicine, 1967