Cigarette Smoke‐Induced Oxidative Stress and TGF‐β1Increase p21waf1/cip1Expression in Alveolar Epithelial Cells
- 1 November 2002
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 973 (1) , 278-283
- https://doi.org/10.1111/j.1749-6632.2002.tb04649.x
Abstract
Abstract:Sustained oxidative stress caused by cigarette smoking induces a chronic inflammatory response, resulting in the destruction of the alveolar cell wall characteristic of emphysema. The loss of tissue may involve the progressive depletion of epithelial cells through inhibition of proliferation leading to cell death. The cell cycle regulator p21waf1/cip1acts as a G1/S and G2/M phase checkpoint regulator. We hypothesize that cigarette smoke‐induced oxidative stress and transforming growth factor beta 1 (TGF‐β1) may inhibit cellular proliferation by p21waf1/cip1in type II alveolar epithelial cells (A549). A significant increase was observed in p21waf1/cip1mRNA expression in A549 cells by cigarette smoke condensate, H2O2, and TGF‐β1. In conclusion, cigarette smoke‐induced oxidative stress and TGF‐β1modulate expression of the cell cycle inhibitor p21waf1/cip1. This may be important in the growth arrest and cell survival of alveolar type II cells in the G1phase.Keywords
This publication has 21 references indexed in Scilit:
- The Cyclin-Dependent Kinase Inhibitor p21 Protects the Lung from Oxidative StressAmerican Journal of Respiratory Cell and Molecular Biology, 2001
- The role of p21CIP1/WAF1 in growth of epithelial cells exposed to hyperoxiaAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2001
- Histone deacetylase inhibitor selectively induces p21 WAF1 expression and gene-associated histone acetylationProceedings of the National Academy of Sciences, 2000
- Exit from G1 and S Phase of the Cell Cycle Is Regulated by Repressor Complexes Containing HDAC-Rb-hSWI/SNF and Rb-hSWI/SNFCell, 2000
- Histone Deacetylase Inhibition Selectively Alters the Activity and Expression of Cell Cycle Proteins Leading to Specific Chromatin Acetylation and Antiproliferative EffectsJournal of Biological Chemistry, 1999
- Oxygen Induces S-Phase Growth Arrest and Increases p53 and p21WAF1/CIP1 Expression in Human Bronchial Smooth-Muscle CellsAmerican Journal of Respiratory Cell and Molecular Biology, 1999
- Apoptosis and DNA damage in type 2 alveolar epithelial cells cultured from hyperoxic rats.American Journal of Physiology-Lung Cellular and Molecular Physiology, 1998
- Altered Regulation of G1 Cyclins in Oxidant-induced Growth Arrest of Lung Alveolar Epithelial CellsPublished by Elsevier ,1996
- p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrestCell, 1994
- Free-radical chemistry of cigarette smoke and its toxicological implications.Environmental Health Perspectives, 1985