A Micromethod for the Detection of TG and TM Cells. Technical Aspects for the Enumeration of Human T-Cell Subpopulations
- 1 January 1982
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 68 (3) , 214-218
- https://doi.org/10.1159/000233100
Abstract
A rosette technique for the detection of human T-lymphocyte subpopulations with Fc receptors for IgG (TG cells) or IgM (Tm cells) has been worked out using minimal amounts of IgG and IgM antibodies. Antibody-coated ox red blood cells (EA) were used as indicator cells. Optimal conditions for rosette enumerations required intimate T-cell-EA contact and incubation of cell mixtures for 3 h at 4°C. The TM-cell level increased after culture of T cells, and TM-cell rosettes were found to be more fragile. The rosette assay was found to be reproducible and thus useful in clinical immunology.Keywords
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