Use of the omp50 Gene for Identification of Campylobacter Species by PCR
Open Access
- 1 May 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (5) , 2301-2305
- https://doi.org/10.1128/jcm.42.5.2301-2305.2004
Abstract
We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and most C. jejuni and C. lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species.Keywords
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