Improvement of the Specificity of an Enzyme-Linked Immunosorbent Assay for Diagnosis of Paracoccidioidomycosis
- 1 April 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 43 (4) , 1944-6
- https://doi.org/10.1128/jcm.43.4.1944-1946.2005
Abstract
In an attempt to improve the specificity of an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paracoccidioidomycosis (PCM), sera from patients with PCM were tested using various approaches, such as sodium metaperiodate antigen (gp43) treatment, a serum absorption process withCandida albicansorHistoplasma capsulatumantigens, and dilution of serum in galactose, the main common epitope among pathogenic fungi. The maximum specificity found in this ELISA was 84%. All of these procedures proved inefficient for eliminating all cross-reacting antibodies and obtaining an ELISA specific for PCM diagnosis.Keywords
This publication has 30 references indexed in Scilit:
- Simplified method for producingParacoccidioidesbrasiliensisexoantigens for use in immunodiffusion testsMedical Mycology, 2003
- Current knowledge on pathogenesis and immunodiagnosis of paracoccidioidomycosis.2000
- 43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplasmosis, or Jorge Lobo's disease.1991
- Purification of the 43 kDa glycoprotein from exocellular components excreted byParacoccidioides brasiliensisin liquid culture (TOM medium)Medical Mycology, 1991
- New experimental criteria for optimization of solid-phase antigen concentration and stability in ELISAJournal of Immunological Methods, 1986
- Detection of Antibodies and Antigenemia in Leukemic Patients with Candidiasis by Enzyme-Linked Immunosorbent AssayThe Journal of Infectious Diseases, 1981
- Enzyme-linked immunosorbent assay (ELISA) for IgM, IgG and IgA class antibodies againstCandida albicansantigens: Development and comparison with other methodsMedical Mycology, 1981
- Development of an enzyme immunoassay for cryptococcal antibodyMycopathologia, 1980
- Enzyme linked immunosorbent assay (ELISA) for IgG and IgE antibodies to protein and polysaccharide antigens of Aspergillus fumigatusClinical and Experimental Allergy, 1979
- The immunology of South-American blastomycosisMycopathologia, 1965