Fractionation of Human Serum Lipoproteins by High Performance Liquid Chromatography. I

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Abstract
Immunologically pure lipoprotein fractions of human serum were subjected to high performance liquid chromatography (HPLC) on a combination system of aqueous gel permeation columns. Each lipoprotein fraction appeared at a distinct elution volume as a single homogeneous peak. Elution was carried out with 0.1 m Tris-HCl buffer solution, pH 7.4, or 0.15 m NaCl solution and no significant differences in elution volume and elution pattern were found. The lipoprotein mixtures (total lipoprotein fraction d< 1.210) of healthy and pathological subjects were subjected to HPLC and showed characteristic elution patterns which could be of diagnostic significance.