Glutamine Assimilation Pathways in Neurospora crassa Growing on Glutamine as Sole Nitrogen and Carbon Source

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Abstract
Neurospora crassa wild-type is almost unable to grow on glutamine as sole nitrogen and carbon source but a GDH-;GS.+-. double mutant strain, lacking NADP-dependent glutamate dehydrogenase and partially lacking glutamine synthetase did grow. Under these conditions, the double mutant had a higher chemical energy content than the wild-type. Enzyme assays and labelling experiments with glutamine indicated that in the double mutant glutamine was degraded to ammonium and to carbon skeletons by glutamate synthase, the catabolic (NADH-dependent) glutamate dehydrogenase and the glutamine transaminase-.omega.-amidase pathway.