Selective reduction of c-myc mRNA in Daudi cells by human beta interferon.

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Abstract
Under normal growth conditions, the human lymphoblastoid cell line Daudi expresses high levels of c-myc mRNA. These cells are also sensitive to growth inhibition by interferons. The levels of mRNA for the c-myc in untreated and human .beta. interferon (IFN-.beta.)-treated Daudi cells by RNA dot-blot and blot-hybridization analysis methods were compared. Using a synthetic oligonucleotide complementary to the human c-myc mRNA as the probe, a > 75% reduction in the c-myc hybridizable poly(A)+ RNA in the IFN-.beta.-treated cells was detected. This reduction in the c-myc mRNA appears to be selective because the level of actin mRNA is not significantly affected by the IFN-.beta. treatment. In addition, neither in vitro translation of mRNA extracted from IFN-.beta.-treatment cells nor in vivo synthesis of cellular proteins in IFN-.beta.-treated cells are quantitatively affected. The selective reduction in the amount of c-myc mRNA in IFN-B-treated Daudi cells may be related to the IFN-induced inhibition of the Daudi tumor cell growth.