A note on the determination of the ester sulphate content of sulphated polysaccharides

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Abstract
A method is described for the analysis of the ester sulphate contents of polysaccharide sulphates of plant and animal origin. The esters are hydrolysed with acid under conditions designed to reduce the rate of formation of ultraviolet-absorbing materials and liberated sulphate is then measured turbidimetrically by a modification of the procedure of Dodgson (1961). Results obtained with the method agreed closely with those obtained by a conventional large-scale gravimetric procedure.