QUANTITATIVE EXTRACTION OF EVANS BLUE LEAKED FROM THE VESSELS IN THE SKIN

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Abstract
The relatively simple and highly reliable extraction method of Evans blue leaked in the skin from the intravascular circulation was described. The method consisted of the following 3 procedures; treatment of the skin with protease, boiling of the hydrolysed tissue with sodium dodecyl sulfate and conversion of sulfonate type of the dye to sulfonic acid type readily extractable with butanol. The method enabled the increased permeability of the vessels to a quantitative evaluation.