NMR study of the interaction of P-450 with 4-methoxypyridine
- 9 February 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 212 (1) , 53-57
- https://doi.org/10.1016/0014-5793(87)81555-7
Abstract
Longitudinal relaxation (T1) measurements for all lines (alpha-CH, beta-CH, O-CH3) in the 4-methoxypyridine 1H-NMR spectrum were used to study the interaction of 4-methoxypyridine with purified microsomal cytochrome P-450 from livers of phenobarbital-treated rats. The paramagnetic contribution to the observed T1(-1) value was determined from its dependence on 4-methoxypyridine concentration. In the P-450-4-methoxypyridine complex the latter is oriented so that the nitrogen of pyridine is directed towards the Fe3+.Keywords
This publication has 8 references indexed in Scilit:
- Magnetic Resonance Study of the Structure and Functions of Cytochrome P45Critical Reviews in Biochemistry, 1986
- Interaction of naphthalene-d8 with cytochromes P-450: a 2H nuclear magnetic resonance studyInorganica Chimica Acta, 1985
- 1H‐NMR study of the interaction of aminopyrine with purified rat liver microsomal cytochrome P‐450FEBS Letters, 1985
- Differential metabolism of acetanilide versus ethoxycoumarin and benzo[a]pyrene by two 3-methylcholanthrene-inducible forms of rat liver cytochrome P-450Archives of Biochemistry and Biophysics, 1983
- Proton NMR study of the deprotonation of axial imidazole ligands in low-spin ferric porphyrin complexesJournal of the American Chemical Society, 1982
- Purification, characterization and regulation of five rat hepatic microsomal cytochrome P-450 isozymesXenobiotica, 1982
- Purification of cytochrome P-450, NADPH-cytochrome P-450 reductase, and epoxide hydratase from a single preparation of rat liver microsomesArchives of Biochemistry and Biophysics, 1980
- [27] Measurement of substrate and inhibitor binding to microsomal cytochrome P-450 by optical-difference spectroscopyPublished by Elsevier ,1978