Soft Agar Colony Formation Assay for in Vitro Chemotherapy Sensitivity Testing of Human Renal Cell Carcinoma: Mayo Clinic Experience

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Abstract
Different samples of human renal carcinoma (206) were tested for in vitro chemotherapy sensitivity using a soft agar colony formation assay similar to that originally described by Salmon et al. Of 159, 80 (50%) evaluable tumor tests showed colony formation in vitro and gave clinical drug sensitivity information. Two-thirds of tumors were resistant to all drugs tested, despite a median number of drugs tested per tumor of 14.5. Tumors 5 (6%) were remarkably sensitive to numerous anticancer drugs in vitro. The most active drugs found in vitro were teniposide, actinomycin D, bleomycin, hydroxyurea, mitoguanazone dihydrochloride, mitomycin C and L-alanosine. Other drugs tested showed low in vitro cytotoxicity.