PSEUDOURIDINE FROM CULTURE MEDIA OF AGROBACTERIUM TUMEFACIENS

Abstract
Cultures of Agrobacterium tumefaciens grown on a synthetic medium containing glucose plus a pyrimidine produced a pyrimidine riboside with unusual chemical properties. This substance was isolated in crystalline form and identified as 5-ribosyluracil (pseudouridine) on the basis of data obtained from ion exchange and paper chromatography, electrophoresis, ultraviolet and infrared spectrophotometry, color reactions, melting point, elementary analysis, hydrazinolysis, and periodate oxidation followed by bromine treatment.