Isolation and expression of rat 1,25-dihydroxyvitamin D3 receptor cDNA.
- 1 February 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (4) , 1005-1009
- https://doi.org/10.1073/pnas.85.4.1005
Abstract
The cDNA for the 1,25-dihydroxyvitamin D3 receptor has been isolated by screening a rat kidney lambda gt11 expression library with a mixture of three previously identified anti-receptor monoclonal antibodies. Protein produced from this cDNA reacts with each of the three monoclonal antibodies but does not react with an antibody specific for the porcine receptor or an irrelevant antibody. In addition, the expressed protein specifically binds 1,25-dihydroxyvitamin D3 but does not bind estradiol, progesterone, testosterone, cortisol, or aldosterone. The nucleotide sequence for this cDNA has been determined and used to deduce the amino acid sequence of the protein. This amino acid sequence contains a region that matches perfectly the partial amino acid sequence determined for the receptor isolated from porcine intestine. This amino acid sequence also closely matches the reported amino acid sequence of the putative DNA-binding finger of the avian 1,25-dihydroxyvitamin D3 receptor.This publication has 14 references indexed in Scilit:
- Molecular Cloning of Complementary DNA Encoding the Avian Receptor for Vitamin DScience, 1987
- Avian and mammalian receptors for 1,25-dihydroxyvitamin D3: in vitro translation to characterize size and hormone-dependent regulation.Proceedings of the National Academy of Sciences, 1987
- Monoclonal antibodies to the porcine intestinal receptor for 1,25-dihydroxyvitamin D3: interaction with distinct receptor domainsBiochemistry, 1986
- VITAMIN D: RECENT ADVANCESAnnual Review of Biochemistry, 1983
- Efficient isolation of genes by using antibody probes.Proceedings of the National Academy of Sciences, 1983
- Development of hybridomas secreting monoclonal antibodies to the chicken intestinal 1 alpha,25-dihydroxyvitamin D3 receptor.Proceedings of the National Academy of Sciences, 1982
- A Cellular Defect in Hereditary Vitamin-D-Dependent Rickets Type II: Defective Nuclear Uptake of 1,25-Dihydroxyvitamin D in Cultured Skin FibroblastsNew England Journal of Medicine, 1981
- Direct chemical synthesis of 1.alpha.,25-dihydroxy[26,27-3H]vitamin D3 with high specific activity: its use in receptor studiesBiochemistry, 1980
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977