Enhancement of arachidonic acid liberation by protein kinase C activator is partially dependent on extracellular Na+ in rabbit platelets
- 28 August 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 254 (1-2) , 29-32
- https://doi.org/10.1016/0014-5793(89)81003-8
Abstract
In [3H]arachidonic acid‐labeled rabbit platelets, pretreatment with phorbol 12‐myristate 13‐acetate (20 nM) or dioctanoylglycerol (20 μM) enhanced [3H]arachidonic acid liberation induced by low concentration of A23187 (150 nM). When extracellular Na2+ was replaced with N‐methyl‐D‐glucamine, the enhancement is reduced by about 50%. Similar synergistic enhancement of the liberation was obtained by using monensin (2–10 μM) or NH4Cl (5–20 mM) in place of protein kinase C activator in combination with A23187. The guanosine 5′‐O‐[3‐thiotriphosphate] (100 μM)‐induced liberation was also enhanced by a rise of extracellular pH (pH 7.0–7.8) in saponin‐permeabilized platelets. These results suggest that the enhancement of arachidonic acid liberation by protein kinase C may partially be mediated by intracellular alkalinization in rabbit platelets.Keywords
This publication has 14 references indexed in Scilit:
- Differential effects of phorbol 12-myristate 13-acetate on GTPγS-induced diacylglycerol formation and arachidonic acid liberation in saponin-permeabilized rabbit plateletsThrombosis Research, 1989
- Role of Na+/H+ exchange in thrombin- and arachidonic acid-induced Ca2+ influx in plateletsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1988
- Protein kinase C enhances Ca2+ mobilization in human platelets by activating Na+/H+ exchange.Published by Elsevier ,1988
- Thrombin and C-kinase activators potentiate calcium-stimulated arachidonic acid release in human plateletsBiochemical Journal, 1987
- Inhibition of Na+/H+ exchange reduces Ca2+ mobilization without affecting the initial cleavage of phosphatidylinositol 4,5‐bisphosphate in thrombin‐stimulated plateletsFEBS Letters, 1987
- Activation of Na+/H+ exchange in human platelets stimulated by thrombin and a phorbol esterBiochemical Journal, 1987
- Diacylglycerol lipase pathway is a minor source of released arachidonic acid in thrombin-stimulated human plateletsBiochemical and Biophysical Research Communications, 1986
- Phorbol esters and oleoyl acetoyl glycerol enhance release of arachidonic acid in platelets stimulated by Ca2+ ionophore A23187.Journal of Biological Chemistry, 1985
- The influence of Na+ on the alpha 2-adrenergic receptor system of human platelets. A method for removal of extraplatelet Na+. Effect of Na+ removal on aggregation, secretion, and cAMP accumulation.Journal of Biological Chemistry, 1983
- A new approach to anti-inflammatory drugsBiochemical Pharmacology, 1979