Activation of Na+/H+ exchange in human platelets stimulated by thrombin and a phorbol ester
- 1 January 1987
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 241 (1) , 301-303
- https://doi.org/10.1042/bj2410301
Abstract
We have investigated changes in cytoplasmic pH (pHi) in activated human platelets, using the fluorescent probe 2,7-biscarboxyethyl-5(6)-carboxyfluorescein. Stimulation of platelets by thrombin or 12-O-tetradecanoylphorbol 13-acetate increased pHi by about 0.11 pH unit above the resting value. This increase in pHi depended on the presence of external Na+ and was inhibited by ethylisopropylamiloride. The data suggest that protein kinase C mediates Na+/H+ exchange in human platelets.This publication has 16 references indexed in Scilit:
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