Note: Identification ofLactobacillus delbrueckiisubspeciesbulgaricusand subspecieslactisdairy isolates by amplified rDNA restriction analysis
Open Access
- 1 November 1998
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 85 (5) , 918
- https://doi.org/10.1046/j.1365-2672.1998.00606.x
Abstract
Amplification and restriction analysis of the 16S rRNA gene (PCR‐ARDRA) was used to identify Lactobacillus delbrueckii dairy isolates and to assign them to its three subspecies (delbrueckii, lactis and bulgaricus). PCR‐ARDRA allowed confirmation of predictions from the DNA database regarding the restriction mapping of the 16S sequence to be verified in practice. PCR‐ARDRA was shown to be a reliable and rapid method for identifying Lact. delbrueckii isolates at the subspecies level and for differentiating this species from Lact. helveticus and Lact. acidophilus.Keywords
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