Continuous centrifugation in virus processing

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Abstract
Ambler's Q/Σ relationship between the throughput of a centrifuge and its mechanical characteristics has been translated from batch data in order to predict operating conditions for continuous centrifugation in virus processing. A Sharples Laboratory Supercentrifuge was converted from batch to continuous operation and a slurry feed device was developed. A correlation between operating conditions for continuous centrifugation and amount of test virus recovered from the feed showed that the Q/Σ values at which high concentrations of the virus were recovered were almost identical to the Q/Σ values for significant virus recovery in batch centrifugation. Titration and gravimetric determinations of the sediments recovered from continuous centrifugation operations indicate that product purity might be influenced by operating conditions.

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