High-Performance Liquid Chromatography of Fat-Soluble Vitamins. I. Determination of Vitamin A-Acetate in Pharmaceutical Preparations and Blood

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Abstract
A reversed phase high-performance liquid chromatography (HPLC) has been developed for the quanititive determination of retinol acetate (vitamin A-acetate) in pharmaceutical formulations and biological materials. The extraction of vitamin A-acetate from capsules and dragees and from blood is performed in a fully automated, electronically controlled extraction apparatus within 3–10 minutes. For reversed phase HPLC a column of LiChrosorb RP18 and methanol as eluent were used. Vitamin A-acetate was separated in less than 1 minute. The detection limit was 1-2 ng. The described methods were proved useful for extraction and determination of vitamin A-acetate in pharmaceutical preparations such as capsules and dragees and in blood, and can be well reproduced with a maximum coefficient of variation of <4%.