Adsorptions of Plasma Proteins and Their Elutabilities from a Polysiloxane Surface Studied by an On-Line Acoustic Wave Sensor

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Abstract
Gold electrodes of thickness-shear mode acoustic wave sensors were modified with poly[(mercaptopropyl)methylsiloxane]. The flow-through adsorption of three major plasma proteins (human serum albumin, fibrinogen, and immunoglobulin G) was detected by acoustic network analysis. The elution of fibrinogen and albumin from coated and unmodified gold surfaces by sodium dodecyl sulfate was studied with respect to different adsorption times and protein concentrations. Both sequential and competitive adsorptions of the three proteins on polymer-modified surfaces of sensors were examined as were simultaneous adsorptions from binary and ternary mixtures. The experimental results confirm that the competitive behaviors of proteins in terms of adsorptive processes are explained by factors other than displacement phenomena.