Ready separation of proteins from nucleoprotein complexes by reversible modification of lysine residues
- 1 October 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 191 (1) , 269-272
- https://doi.org/10.1042/bj1910269
Abstract
Modification of proteins with citraconic anhydride altered the electrostatic relationship between cationic epsilon-NH3+ groups of lysine residues of proteins and anionic phosphate groups of nucleic acids, thereby destabilizing the nucleoprotein complex. This procedure facilitated the separation of proteins from nucleic acids at pH4-4.2. The modifying groups were then deacylated from the proteins under acidic conditions (pH3-6) at 30 degrees C.This publication has 10 references indexed in Scilit:
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