Th1/Th2 response profiles to the major allergens Cry j 1 and Cry j 2 of Japanese cedar pollen
- 1 October 1996
- Vol. 51 (10) , 732-740
- https://doi.org/10.1111/j.1398-9995.1996.tb02118.x
Abstract
Cry j 1 and Cry j 2 are known to be the major allergens of Japanese cedar pollen. A comparative study was carried out on the immune responses to stimulation with Cry j 1 and Cry j 2 in 24 symptomatic patients and six nonallergic subjects. In T-cell proliferation assays, mean stimulation indexes (SI) were 10.6 for Cry j 1 and 11.7 for Cry j 2 stimulation, respectively, in the allergic patients. Two of the nonallergic subjects showed strong T-cell proliferation to both allergens, while the remainder did not. All the allergic subjects (17/17) showed high titers of anti-Cry j 1 IgE antibody at a mean value of 165 U/ml, whereas only 64% responded to Cry j 2 with low titers at a mean value of 26 U/ml. Nonallergic subjects did not respond with IgE production. Allergic subjects were further examined for their cytokine production profiles. All allergic subjects tested (16/16) produced high levels of interferon-gamma (IFN-γ) in response to Cry j 1 with a mean value of 918 pg/ml, while only five subjects showed significant elevation of IFN-γ production in response to Cry j 2 with a mean value of 679 pg/ml. The remainder produced small amounts of IFN-γ. Cry j 1 induced higher levels of interleukin (1L)-10 gene expression than did Cry j 2 stimulation, while both allergens induced IL-4 expression at a similar level. The IL-12 p35 gene was constitutively expressed, whereas the IL-12 p40 gene expression in Cry j 1-stimulated cells was elevated eightfold over that of nonstimulated cells. Increased expression of the IL-12 p40 gene was negligible in Cry j 2-stimulated cells. Thus, Cry j 1 stimulated mixed features of Th1 and Th2-like responses, while Cry j 2 played a minor role in inducing IgE production and cytokine (IFN-γ, IL-10, and IL-12) production, except for IL-2 production and strong T-cell proliferative activity. Therefore, it was concluded that Cry j 1 is the more important allergen, and that T-cell proliferation assays do not necessarily reflect the level of allergenicity.Keywords
This publication has 37 references indexed in Scilit:
- Cry j I, a major allergen of Japanese cedar pollen, has pectate lyase enzyme activityAllergy, 1995
- Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollenFEBS Letters, 1994
- Cloning and Sequencing of cDNA Coding for Cry j I, a Major Allergen of Japanese Cedar PollenBiochemical and Biophysical Research Communications, 1994
- House dust mite allergy: from T-cell epitopes to immuno-therapyEuropean Journal of Clinical Investigation, 1993
- Epitopes on Cry j I and Cry j II for the human ige antibodies cross-reactive between Cupressus sempervirens and Cryptomeria japonica pollenMolecular Immunology, 1993
- High sensitivity of human melanoma cell lines to the growth inhibitory activity of mycoplasmal arginine deiminase in vitroMelanoma Research, 1992
- T cell responses to the purified major allergens from the house dust mite Dermatephagoides pteronyssinusJournal of Allergy and Clinical Immunology, 1992
- Antigenic Analyses of Sugi Basic Protein by Monoclonal Antibodies; I. Distribution and Characterization of B-Cell-Tropic Epitopes of Cry j I MoleculesInternational Archives of Allergy and Immunology, 1992
- N‐terminal amino acid sequence of a major allergen of Japanese cedar pollen (Cry j I)FEBS Letters, 1988
- Two types of mouse helper T-cell clone: Implications for immune regulationImmunology Today, 1987