Improved method for immunoperoxidase detection of membrane antigens in frozen sections.

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Abstract
The visualization of membrane antigens in frozen sections using metal-enhanced 3,3''diaminobenzidine (DAB) was investigated. Particular attention was paid to the degree of reaction enhancement, the disruptive effect on morphological detail, and the ease of the techniques. The best result was obtained using nickel-modified DAB at pH 6.0 to develop the peroxidase enzyme, with further enhancement in cobalt chloride at neutral pH. Silver methenamine enhancement is also possible but can give to non-specific or background staining.