Phosphorylation by Cyclin B-Cdk Underlies Release of Mitotic Exit Activator Cdc14 from the Nucleolus
- 23 July 2004
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 305 (5683) , 516-519
- https://doi.org/10.1126/science.1099402
Abstract
Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release (FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEARnetwork promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.Keywords
This publication has 21 references indexed in Scilit:
- Separase Regulates INCENP-Aurora B Anaphase Spindle Function Through Cdc14Science, 2003
- The Role of the Polo Kinase Cdc5 in Controlling Cdc14 LocalizationMolecular Biology of the Cell, 2003
- Targets of the cyclin-dependent kinase Cdk1Nature, 2003
- The Cdc14 Phosphatase and the FEAR Network Control Meiotic Spindle Disassembly and Chromosome SegregationDevelopmental Cell, 2003
- Division of the Nucleolus and Its Release of CDC14 during Anaphase of Meiosis I Depends on Separase, SPO12, and SLK19Developmental Cell, 2003
- Role of Polo-like Kinase CDC5 in Programming Meiosis I Chromosome SegregationScience, 2003
- A non-proteolytic function of separase links the onset of anaphase to mitotic exitNature Cell Biology, 2003
- Separase, Polo Kinase, the Kinetochore Protein Slk19, and Spo12 Function in a Network that Controls Cdc14 Localization during Early AnaphaseCell, 2002
- The nucleolus: the magician's hat for cell cycle tricksCurrent Opinion in Cell Biology, 2000
- Exit from Mitosis Is Triggered by Tem1-Dependent Release of the Protein Phosphatase Cdc14 from Nucleolar RENT ComplexCell, 1999