Rapid Spectrophotometric Assay of Cephradine and its Dosage Forms

Abstract
A new spectrophotometric method to determine cephradine based on its reaction with acetylacetone-formaldehyde reagent (pH 4.3) is proposed. The yellow chromophore shows an absorption maxima at 400 nm, having a molar absorptivity coefficient of 2.59. 103 L mol−1 cm−1. Optimization of the reaction conditions has been investigated. Obediance to Beer's law (0–110 mog per ml) permitted the assay of cephradine in its dosage forms. The method has been compared with the official method and found to be simple, artwrate (t-test) and reproducible (F-test).