Isolated perfused rat small bowel--technic, studies of viability, glucose absorption

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Abstract
A procedure is described for surgically isolating and artificially perfusing the small intestine of the rat. Washed bovine red blood cells suspended in an "artificial plasma" are employed as the perfusate. The viability of the preparation for at least 1-2 hr. is demonstrated by steady-state O2 and glucose consumption, constancy of blood flows, absorption of glucose against a concentration gradient, and histological studies. The technic has wide applications in the study of intestinal absorption, secretion, and metabolism.