Supercoiled induced transition to the Z-DNA conformation affects the ability of a d(CG/GC)12sequence to be organized into nucleosome-cores
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (21) , 8899-8918
- https://doi.org/10.1093/nar/15.21.8899
Abstract
Nucleosome-cores were reconstituted by the salt-dialysis method onto closed circular pDHg16 DNA which contains a d(CG/GC)12 sequence. Alternating d(CG/GC)n sequences form left-handed Z-DNA readily when contained in negatively supercoiled DNA. We have investigated the ability of the d(CG/GC)12 sequence to be organized into nucleosome-cores when stabilized as Z-DNA through negative supercoiling. We have found that nucleosome assembly at the d(CG/GC)12 insert is prevented when the sequence 1s stable in the Z-conformation but it is not affected at all when the sequence adopts the right-handed B-form.Keywords
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