Matrix Solid Phase Dispersion Isolation and Liquid Chromatographic Determination of Five Benzimidazole Anthelmintics in Fortified Beef Liver

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Abstract
A multlresidue method for Isolation and liquid chromatographic determination of 5 benzimidazole anthelmintics (thiabendazole, oxfendazole, mebendazole, albendazole, and fenbendazole) in beef liver tissue is presented. Blank or benzlmldazole-fortlfled liver samples (0.5 g) were blended with octadecylsilyl derlvatlzed silica packing material (C18, 18% load, endcapped, 2 g). A column made from the C18/ liver matrix was first washed with hexane (8 ml_), following which the benzlmldazoles were eluted with acetonltrile. The acetonltrlle extract was then passed through an activated alumina column. The eluate contained benzimidazole analytes that were free from Interfering compounds as determined by UV detection (photodlode array, 290 nm). Correlation coefficients of standard curves for Individual benzlmldazoles Isolated from fortified samples, using internal standardization, were linear (0.996 ± 0.002 to 0.999 ± 0.001) with average relative percentage recoveries from 62.0 ± 6.7 to 86.8 ± 8.6% for the concentration range (100- 3200 ng/g) examined. The interassay variability was 7.0 ± 4.1 to 12.9 ± 10.2% with an Intra-assay variability from 2.2 to 4.0%.