Kinetics of Conformational Change of Troponin-C Induced by Proton Binding or Removal in the Absence of Calcium Ions
- 1 February 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 81 (2) , 277-283
- https://doi.org/10.1093/oxfordjournals.jbchem.a131456
Abstract
The kinetics of the conformational change of troponin-C induced by binding or removal of protons was studied by a stoppd-flow pH-jump spectrofluorometric method. In the pH-down experiment (to investigate the kinetics of conformational change from the deprotonated state to the protonated state), a single first-order reaction with a rate constant and amplitude of 1.75–2.4 sec−1 and around 10%, respectively, was observed. On the other hand, two firstorder reactions with rate constants of 0.84–1.6 sec−1 and 0.08–0.4 sec−1 were observed in the pH-up experiment, the total amplitudes of these reactions being around 10–20%. The pH dependences of the rate constants of these reactions were analyzed in terms of a three-species mechanism.Keywords
This publication has 2 references indexed in Scilit:
- A quantitative treatment of the kinetics of the folding transition of ribonuclease ABiochemistry, 1976
- Kinetics of Conformational Change of Troponin C Induced by Calcium1The Journal of Biochemistry, 1976