ELECTRON MICROSCOPIC RADIOAUTOGRAPHY USING A COMBINATION OF PHENIDON DEVELOPER AND DOMESTIC EMULSION

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Abstract
A routine procedure for EM radioautography in combination with phenidon developer and the domestic emulsion, Sakura NR-H2, was developed. Good stable radioautograms were not obtained by simply developing the emulsion coated grids with phenidon developer at any temperature, but the development of these grids at 18.degree. C for 1 min after 30-45 s Au latensification in freshly prepared gold thiocyanate gives satisfactory results. Some EM radioautograms developed by this method are presented. The addition of the anionic surfactant, dioctyl sodium sulfosuccinate, to the emulsion was very effective for preventing the burst of emulsion and in obtaining a uniform spread of the emulsion for the wire loop method. Radioautograms obtained with this method are suitable for quantitative EM radioautographic studies. Details of this method are introduced. [Dunn and Potter DBA mouse mastocytoma cells, rabbit bone marrow cells, human cervical carcinoma HeLa cells and mouse cornea cells were used to demonstrate the utility of this method].

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