The human leukocyte test system. V. DNA synthesis and mitoses in PHA-stimulated 3-day cultures.
- 25 August 1975
- journal article
- Vol. 28 (4) , 295-302
Abstract
In human leukocyte cultures st up with TC medium 199,DNA synthesis and mitotic indices were analysed by means of 3H-thymidine autoradiography and cell counting. DNA synthesis starts at around 28 hrs. The frequencies of labelled cells rise slowly and reach a maximum of around 24%. The first mitoses appear at around 38 hrs but up to 49 hrs only very few mitoses can be seen. After that time the mitotic indices rise and reach values of up to 11% cultivation in the presence of BudR for 72 hrs and staining with Hoechst 33258 stain revealed that first, second and third mitoses occur together in the cultures at this time. Irradiation of whole blood and cultivation for 72 hrs leads to mitoses containing dicentric and ring chromosomes with and without fragments, to interphases with micronuclei, to premature chromosome condensations (PCC) and to polyploid mitoses indicating that at this time first and further mitoses are present.This publication has 14 references indexed in Scilit:
- The human leukocyte test systemMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
- The human leukocyte test systemMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- Microfluorometric Detection of Deoxyribonucleic Acid Replication in Human Metaphase ChromosomesProceedings of the National Academy of Sciences, 1973
- DNA synthesis and distribution of first and second divisions in 72-h-cultures of human leukocytesCellular and Molecular Life Sciences, 1972
- Factors influencing chromosome aberration yields in the human peripheral leukocyte systemMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1969
- Studies on in vitro lymphocyte proliferation in cultures synchronized by the inhibition of DNA synthesis: I. Variability of S plus G2 periods of first generation cellsExperimental Cell Research, 1969
- Time-course of DNA synthesis in human leukocyte culturesExperimental Cell Research, 1963
- An improved nutrient solution for diploid Chinese hamster and human cell linesExperimental Cell Research, 1963
- DNA synthesis and mitosis in cultures of human peripheral leukocytesExperimental Cell Research, 1962
- Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium.,Experimental Biology and Medicine, 1950