Radiolabeling revisited: metabolic labeling with35S‐methionine inhibits cell cycle progression, proliferation, and survival
- 1 March 2000
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 14 (3) , 448-454
- https://doi.org/10.1096/fasebj.14.3.448
Abstract
Metabolic labeling of cells with low-energy beta-emitting radioisotopes such as [35S]methionine is often used to follow the biosynthesis, maturation, and degradation of proteins in vivo. Such techn...Keywords
This publication has 13 references indexed in Scilit:
- Accurate quantitation of protein expression and site-specific phosphorylationProceedings of the National Academy of Sciences, 1999
- Cyclin-dependent Kinases Are Inactivated by a Combination of p21 and Thr-14/Tyr-15 Phosphorylation after UV-induced DNA DamageJournal of Biological Chemistry, 1996
- Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl.The Journal of Experimental Medicine, 1995
- p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts.Proceedings of the National Academy of Sciences, 1995
- Elevated levels of wild-type p53 induced by radiolabeling of cells leads to apoptosis or sustained growth arrestCurrent Biology, 1995
- DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.Genes & Development, 1994
- Evidence for M-Phase-Specific Modification of a Gap Junction ProteinPublished by Springer Nature ,1994
- Radiolabeling of DNA can induce its fragmentation in HL-60 human promyelocytic leukemic cellsExperimental Cell Research, 1992
- Malignant transformation induced by incorporated radionuclides in BALB/3T3 mouse embryo fibroblasts.Proceedings of the National Academy of Sciences, 1982
- Production of large numbers of mitotic mammalian cells by use of the reversible microtubule inhibitor NocodazoleExperimental Cell Research, 1980