Abstract
The hydrolytic activity of 40 pure cultures of bacteria on triolein, as indicated by increased titratable acidity, has been recorded. Schiff and Kreis tests have been made on similar samples of triolein acted upon by pure cultures of bacteria, and the results of these tests are compared with the "oxidase reaction" of the individual cultures.The results indicate that most of the Gram-negative lipolytic organisms also oxidize the fat, and that there appears to be a definite relation between the "oxidase reaction" of a bacterial colony and the ability of the organisms to produce oxidative rancidity.

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