Identification of Non-Heme Diiron Proteins That Catalyze Triple Bond and Epoxy Group Formation
- 8 May 1998
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 280 (5365) , 915-918
- https://doi.org/10.1126/science.280.5365.915
Abstract
Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Δ12 acetylenic bond in 9-octadecen-12-ynoic acid and the Δ12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana , the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non–heme iron that have histidine-rich motifs.Keywords
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