V genes of oxazolone antibodies in 10 strains of mice

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Abstract
One pair of V genes (Vx45.1 and V11) code for a great portion of phenyloxazo‐lone (anti‐phOx) antibodies in 10 strains of mice. This combination replaces the first‐known major combination VHOx1‐VxOx1 in some strains, and is important in most strains. C57BL/10 and SJL mice have an additional subset of antibodies encoded by genes Vx45.1 and V13 (a relative of V11). All three genes involved (Vx45.1, V11 and V13) have „allelic”︁ variation. Four alleles of V11 were found, one in Igh haplotypes a, c and g, the second in haplotypes d, j and n, the third in b, and the fourth in f. The most distant alleles d,j,n and f had 10 nucleotide differences out of 429 determined (97.7% homology). Only one allele of the V13 gene was found from anti‐phOx hybridomas but two others have been published. Three alleles of the Vx45.1 gene were found; one in NZB mice (Igx haplotype b) another in CE (haplotype f), and the third in eight strains including representatives of three Igx haplotypes (a, c and e). The three alleles had >99.0% homology. The V11 and V13 genes that code for anti‐phOx antibodies in C57BL/10 and SJL mice were different from the related genes found from the C57BL/10 germ line. C57BL/10 mice must have a chromosome bearing two V11 and two V13 genes. RF mice were found to have two V11 genes, and both code for anti‐phOx antibodies. Our data show that the majority of antibodies in the anti‐phOx response are encoded by the same restricted collection of V genes in most mouse strains. Antibody responses appear to be no less heritable than other functions of the body.

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