Protein synthesis in cucumber mosaic virus-infected tobacco leaves, pre-infected cells and protoplasts

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Abstract
Polypeptide synthesis has been investigated in cucumber mosaic virus-infected tobacco by radiolabelling and polyacrylamide gel electrophoresis. In addition to the coat protein polypeptide of molecular weight 27,000, others of higher molecular weightca. 120,000 to ≥160,000 (probably two) and possibly one ofca. 32,000–34,000 were detected in the particulate fractions only. A polypeptide ofca. 53,000–57,000 was particularly evident in leaves systemically infected using a differential temperature treatment. Two polypeptides of low molecular weight,ca. 23,000–24,000 and 21,000, were only observed in pre-infected cells and protoplasts and probably represent coat protein degradation products.