Stimulation of rat renal Na+, K+-ATPase activity by thyroid hormones

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Abstract
The effect of thyroid hormones (T4, T3 and reverse T3) on rat renal Na+, K+‐ATPase activity was investigated by a cytochemical technique. T3 caused stimulation of Na+, K+‐ATPase activity in the renal medulla but not in the renal cortex. There was a peak in enzyme activity after cultured renal segments had been exposed to T3 for 11 min and this time of maximal stimulation did not vary with the concentration of T3. A rectilinear response in Na+, K+‐ATPase activity was observed over T3 concentration range 10 pmol l−1 to 100 nmol l−1; at higher T3 concentrations, Na+,K+‐ATPase activity was inhibited. The enzyme response was totally blocked by specific T3 antiserum. Addition of T4 and reverse T3 (100 fmol l−1 − 1 mmol l−1) failed to stimulate Na+,K+‐ATPase activity in any part of the kidney. Plasma (neat and diluted 1:10) stimulated the enzyme in parallel with the dose response curve and the stimulatory effect was abolished by prior addition of specific T3 antiserum.

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