SEQUENCE OF CYANOGEN BROMIDE FRAGMENTS D AND E OF B. AMYLOLIQUEFACIENS ALPHA AMYLASE

Abstract
Alpha amylase (Sigma type IIA) was cleaved by cyanogen bromide. The sequences of two fragments, D which contains 88 amino acids and E which contains 56, are presented. By applying the Chou‐Fasman rules, D is predicted to have 32% beta sheet and 37% alpha helix, and E is predicted to exhibit 11% beta sheet and 71% alpha helix.