A new immunoperoxidase assay for Lolium perenne‐specltic IgE in serum based on the biotin/avidin system (BAS)
- 1 July 1984
- journal article
- research article
- Published by Wiley in Clinical and Experimental Allergy
- Vol. 14 (4) , 373-378
- https://doi.org/10.1111/j.1365-2222.1984.tb02218.x
Abstract
A new solid-phase immunoassay based on the biotin/avidin system (BAS) for measuring serum L. perenne (LP)-specific IgE antibody is described. LP-specific IgE was assayed by the BAS assay and RAST [radioallergosorbent test] for comparison in the sera of 32 normal asymptomatic [human] subjects RAST-negative for LP and of 26 subjects with hay fever and RAST-positive for LP. The specificity of the BAS assay for LP-specific IgE was demonstrated by absorption experiments. An overall agreement of 91% (53/58) was observed between the BAS and RAST; a high correlation (r = 0.87, P < 0.001) was found between the LP-specific IgE determined by the 2 methods. The advantages of the BAS assay as compared to the RAST and classical ELISA [enzyme-linked immunosorbent assay] are discussed.This publication has 7 references indexed in Scilit:
- PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATIONJournal of Histochemistry & Cytochemistry, 1974
- Diagnostic tests in ragweed hay fever: A comparison of direct skin tests, IgE antibody measurements, and basophil histamine releaseJournal of Allergy and Clinical Immunology, 1973
- Radioimmunosorbent assay of allergensJournal of Allergy and Clinical Immunology, 1972
- The radioallergosorbent test in the in vitro diagnosis of multiple reaginic allergyJournal of Allergy and Clinical Immunology, 1971
- Clinical significance of specific IgE to common allergensClinical and Experimental Allergy, 1971
- In vitro Diagnosis of Atopic AllergyInternational Archives of Allergy and Immunology, 1971
- Coupling of enzymes to proteins with glutaraldehydeImmunochemistry, 1969