Plant cell growth and differentiation may involve GAP regulation of Rac activity
Open Access
- 23 June 1999
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 453 (3) , 341-345
- https://doi.org/10.1016/s0014-5793(99)00750-4
Abstract
Two Rac GTPase cDNAs, LjRac1 and LjRac2, were identified in the legume Lotus japonicus. Two‐hybrid screening with dominant‐constitutive mutations in the two Rac GTPases target three plant cDNAs, LjRacGAP1, LjRacGAP2 and LjRacGAP3, that encode putative GTPase activating proteins of Rho‐GTPase subfamily members. Employing Rac antiserum, purified recombinant LjRac GTPases and recombinant LjRacGAP1, for ligand overlay assays, in vitro GAP affinity assays and GTPase activation, we confirmed that eukaryote Rac/RacGAP interplay is conserved in plants. In this investigation we have developed some tools that can be used to characterize the role of enhanced LjRac2 expression in developing root nodules.Keywords
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