Chemical Modification of Natural Human Tumor Necrosis Factor‐α with Polyethylene Glycol Increases Its Anti‐tumor Potency
Open Access
- 1 January 1994
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 85 (1) , 9-12
- https://doi.org/10.1111/j.1349-7006.1994.tb02879.x
Abstract
Natural human tumor necrosis factor‐α (TNF‐α) was chemically modified with an active ester of monomethoxy polyethylene glycol (PEG). The molecular weight of PEG‐modified TNF‐a depended on the reaction time as well as the initial molar ratio of PEG to TNF‐α. The specific activity of modified TNF‐α was gradually reduced with increase in the degree of PEG‐modification, but the plasma half‐life of TNF‐α was increased by up to 40‐fold. Modified TNF‐α showed approximately 100 times greater anti‐tumor potency than unmodified TNF‐α. Covalent attachment of PEG to TNF‐α thus increased the bioavailability of TNF‐α, and may facilitate its potential therapeutic use.Keywords
This publication has 22 references indexed in Scilit:
- Antitumor Activity of a Novel Chimera Tumor Necrosis Factor (TNF-STH) Constructed by Connecting rTNF-S with Thymosin β4 Against Murine Syngeneic TumorsJournal of Immunotherapy, 1991
- Phase I study of recombinant human tumor necrosis factor ? in advanced malignant diseaseCancer Immunology, Immunotherapy, 1989
- Evaluation of recombinant human tumor necrosis factor by scheduled intratumoral administration in mice bearing transplantable tumors.The Tohoku Journal of Experimental Medicine, 1989
- Chemical modification of recombinant interleukin 2 by polyethylene glycol increases its potency in the murine Meth A sarcoma model.Proceedings of the National Academy of Sciences, 1987
- PROPOSAL OF STANDARDIZED METHODS AND REFERENCE FOR ASSAYING RECOMBINANT HUMAN TUMOR NECROSIS FACTORJapanese Journal of Medical Science and Biology, 1986
- Immune Responses to Polyethylene Glycol Modified L-Asparaginase in MiceInternational Archives of Allergy and Immunology, 1985
- Antitumor activity of murine tumor necrosis factor (TNF) against transplanted murine tumors and heterotransplanted human tumors in nude miceInternational Journal of Cancer, 1984
- Modification of E. coli L-asparaginase with polyethylene glycol: Disappearance of binding ability to anti-asparaginase serumBiochemical and Biophysical Research Communications, 1978
- An endotoxin-induced serum factor that causes necrosis of tumors.Proceedings of the National Academy of Sciences, 1975
- An enzymic method for the trace iodination of immunoglobulins and other proteinsBiochemical Journal, 1969