Structures of sulfated oligosaccharides isolated from the respiratory mucins of a non-secretor (O, Lea+b-) patient suffering from chronic bronchitis
- 1 January 1997
- journal article
- Published by Springer Nature in Glycoconjugate Journal
- Vol. 14 (1) , 113-125
- https://doi.org/10.1023/a:1018525318185
Abstract
Mucin glycopeptides were prepared from the respiratory mucus of a non-secretor, chronic bronchitic patient with blood group O, Lea+b-. Oligosaccharides were released by alkaline borohydride treatment and purified by anion-exchange chromatography, size-exclusion chromatography and high performance anion-exchange chromatography. Structural studies employed 400-MHz 1H-NMR spectroscopy and matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Nine monosulfated oligosaccharides ranging in size from tetra- to hexasaccharide, were fully characterized in this study. The sulfate group occurs either on the C-3 of a terminal galactose residue or on the C-6 of a N-acetylglucosamine residue. In keeping with the non-secretor status of the patient, no structure with an (α1-2)-linked fucose residue was found. Five of the structures had fucose present in (α1-3)-linkage in the X determinant, while only one oligosaccharide (compound 7b) was seen with fucose (α1-4)-linked in the Lea determinant. Eight structures isolated from the mucins of the non-secretor patient had not been found previously in the respiratory mucins; they are listed below.Keywords
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