Transducin‐mediated, isoform‐specific interaction of recombinant rat nucleoside diphosphate kinases with bleached bovine retinal rod outer segment membranes
Open Access
- 1 July 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 389 (2) , 186-190
- https://doi.org/10.1016/0014-5793(96)00575-3
Abstract
The properties of the binding of recombinant rat nucleoside diphosphate (NDP) kinase isoforms a and β (NDP kinase α and β respectively) to bleached bovine retinal rod outer segment (ROS) membranes were investigated. It was found that: (1) both NDP kinase isoforms interacted with ROS membranes in a pH‐, cation‐ and GTPγS‐dependent manner; (2) the retinal G‐protein transducin was an obligatory factor for the interaction; (3) the apparent affinity of NDP kinase a for ROS membranes was about 100‐fold higher than that of NDP kinase β; and (4) an α‐isoform‐specific peptide, corresponding to the sequence of the N‐terminal third (variable region), had the ability to displace bovine NDP kinase from ROS membranes. The results suggest the possible involvement of NDP kinases in cellular regulation via interaction with G‐proteins and provide a structural basis for the possible differential roles of mammalian NDP kinase isoforms in the cell.Keywords
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