Buffers Can Modulate the Effect of Sonication on Egg Lecithin Liposomes

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Abstract
When model membranes are prepared by ultrasonic Irradiation of polyunsaturated phospholipids. radical production can induce a partial degradation of the polyunsaturated fatty acyl chains and the formation of lipid hydroperoxides. A suitable buffer employed during liposome preparation. like Hepes or Tris, is able to exhibit a protective effect against lipid peroxidalion. Heprs has been found to be the most effective. a 10mM concentration provides a 70% protection after 30min sonication. Tris. in the same conditions, exhibits a 50% protection. These findings may be explained on the basis of the rate constants of these organic buffers with hydroxyl radicals.