DEAGGREGATION OF eIF4E INDUCED BY mRNA 5′ CAP BINDING
- 1 April 2005
- journal article
- Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 24 (5-7) , 507-511
- https://doi.org/10.1081/ncn-200061784
Abstract
All eukaryotic mRNAs contain a 5′ terminal cap structure, which consists of 7-methylguanosine linked by a 5′-5′ triphosphate bridge to the first transcribed nucleoside (m7GpppN). Specific recognition of the cap by the eukaryotic initiation factor eIF4E plays a key role in regulation of translation initiation as a rate-limiting step. Using dynamic light scattering (DLS), the apo-form of murine eIF4E (33–217) was shown to aggregate. After addition of m7GTP, progressive deaggregation with the time of incubation in the presence of the cap analogue has been observed.Keywords
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