Inhibition of Protein Synthesis by Didemnins: Cell Potency and SAR

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Abstract
Synthetic and naturally occurring didemnins are potent and specific inhibitors of protein synthesis in vitro. Structure−activity analysis indicates a requirement for the intact macrocycle; however, the smaller ring size represented by the didemnin analogue, tamandarin A, is equipotent to didemnin B. Replacement of the N,O-dimethyltyrosine by a N-methylphenylalanine or N-methylleucine residue is also well-tolerated. The rank order for inhibition of protein synthesis in vitro appears to be retained in MCF-7 cells, albeit at much higher potency. This increase in potency is explained for the first time by data indicating that MCF-7 cells can accumulate didemnin B up to 2−3 orders of magnitude compared to the growth medium.