FURTHER STUDIES ON THE REGULATION OF AMINO SUGAR METABOLISM IN BACILLUS SUBTILIS

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Abstract
Glucosamine 6-phosphate deaminase [2-amino-2-deoxy-D-glucose 6-phosphate ketol-isomerase (deaminating), EC 5.3.1.10] of B. subtilis was partially purified. Its Km is 3.0m[image]. Extracts of B. subtilis contain N-acetylglucosamine 6-phosphate deacetylase (Km T.4 m[image]), glucosamine 1 -phosphate acetylase and amino sugar kinases (EC 2.7.1.8 and 2.7.1.9). Glucosamine 6-phosphate synthetase (L-glutamine-D-fructose 6-phosphate aminotransferase, EC 2.6.1.16) is repressed by growth of B. subtilis in the presence of glucosamine, N-acetylglucos-aniine, N-propionylglucosamine or N-formylglucosamine. Glucosamine 6-phosphate deaminase and N-acetylglucosamine 6-phosphate deacetylase are induced by N-acetylglucosamine. Amino sugar kinases are induced by glucose, glucosamine and N-acetylglucosamine. The synthesis of glucosamine 1-phosphate acetylase is unaffected by amino sugars. Glucose in the growth medium prevents the induction of glucosamine 6-phosphate deaminase and N-acetylglucosamine 6-phosphate deacetylase caused by N-acetylglucosamine; glucose also alleviates the repression of glucosamine 6-phosphate synthetase caused by amino sugars. Glucosamine 6-phosphate deaminase increases in bacteria incubated beyond the exponential phase of growth. This increase is prevented by glucose.